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Raw no. of sequenced reads
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21,656,040
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No. of reads after quality control
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17,257,056
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Total assembly length [bp]
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14,027,954
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Q20 [%]a
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89.52
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Proportion of Ns [%]
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0.02
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GC content [%]
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39.30
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Mean abundance [k-mers] (min – max)b
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16.17 (3 – 165)
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No. of transcripts
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18,080
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No. of singleton contigs
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11,514
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No. of isogroups
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1445
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No. of unigenes
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16,082
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Mean no. transcripts per isogroup (min – max)
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2.38 (2 – 5)
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Mean length of transcripts [bp] (min – max)
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775.89 (200 – 5412)
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N50c [bp]
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1084
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No. of transcripts with BLASTX hitsd
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8289
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No. of transcripts with GO annotationsd
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7070
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No. of transcripts with EC/KEGG annotationsd
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2806
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No. of GO annotated transcripts with tag hitsd, e
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4451
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- aPhred Quality Score
- bMean coverage of k-mers that support a k + 1 length (= 32 bp) of a transcript
- cminimal length of the set of transcripts that makes up half of the total assembly length
- dall BLASTX hits with e < 10− 5 and a sequence similarity of > 55%
- etranscripts that where a best BLASTN hit (bit score > 40) for DeepSuperSAGE tags
